1432 pcDNA3 flag MAGI1c

LOCUS       1432 pcDNA3 flag MAGI1c               9835 bp ds-DNA     circular SYN 07-MAY-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    1432 pcDNA3 flag MAGI1c
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9835)
  AUTHORS   Vazquez F, Grossman SR, Takahashi Y, Rokas MV, Nakamura N, Sellers 
            WR
  TITLE     Phosphorylation of the PTEN tail acts as an inhibitory switch by 
            preventing its recruitment into a protein complex.
  JOURNAL   J Biol Chem. 2001 Dec 28. 276(52):48627-30.
  PUBMED    11707428
REFERENCE   2  (bases 1 to 9835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 7, 2019 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9835
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        173..552
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        553..756
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     707..727
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     801..820
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        801..819
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             845..868
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             872..895
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(5326..5344)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5327..5344)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5364..5381)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5370..5594
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5640..6068
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5727..5746)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5937..5958
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6077..6097)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6082..6411
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6262..6397
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6324..6343
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6478..7272
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6532..6551)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7142..7161
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7446..7567
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7483..7502)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7537..7556
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7616..7632)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7616..7632)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7629..7651)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7640..7656
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7664..7694)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7709..7730
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7847..7864)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8018..8606)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8098..8117)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8777..9637)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9400..9419
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9638..9742)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9817..1)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
       61 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
      121 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
      181 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      241 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      301 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      361 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      421 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      481 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      541 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      601 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      661 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      721 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
      781 gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc ttctcatcat
      841 gggcgactat aaggatgacg atgacaaggg cgattacaaa gacgatgacg ataaatcgaa
      901 agtgatccag aagaagaacc actggactgg ccgcgttcac gagtgcaccg tgaagcgggg
      961 accccagggc gagctggggg tgacggtcct ggggggcgcg gagcatgggg agtttccgta
     1021 cgtgggggcg gtggcggcgg ccgaggcggc ggggcttccc ggcggtggcg aggggccgaa
     1081 gctggccgaa ggtgagctgc tgctggaggt gcagggggtc cgggtgtccg gcttgccccg
     1141 ctatgacgtg ctgggagtca tcgacagctg caaggaggcc gtcaccttca aagccgtcag
     1201 acaaggagga aggctcaaca aggacctacg acatttcctc aaccaacggt tccagaaggg
     1261 gtctccagat catgagctcc agcagaccat aagggacaac ctctaccgcc atgctgtgcc
     1321 ttgcacaacc cggtctccca gagaaggaga agtgcctggc gtggattaca gctttctgac
     1381 tgtgaaggag ttcttggacc tcgagcagag cgggaccctg ttggaagtcg gcacctatga
     1441 aggaaactat tatgggacac ccaaacctcc cagccagcca gtcagtggga aagtgatcac
     1501 gacggatgcc ttgcacagcc tgcagtctgg ctccaaacag tcgaccccta agcgaacaaa
     1561 gtcctacaat gatatgcaaa atgctggcat agtccacccg gagaatgagg aggaggagga
     1621 tgtccctgaa atgaacagta gctttacagc cgactctgga gaccaggacg agcacactct
     1681 ccaagaagca acgctcccac ctgtgaatag tagcatcctc gctgctccca tcacggaccc
     1741 ttctcagaag ttccctcagt acctacctct ttctgcagag gataatttag gtcctctacc
     1801 tgaaaactgg gagatggcct atactgaaaa tggagaagtc tatttcatag accacaacac
     1861 gaaaacaaca tcatggttag accctcggtg cctgaacaaa cagcagaagc ctctggaaga
     1921 atgtgaagat gatgagttgc ctgctggctg ggaaaagatt gaagaccctg tctacggtgt
     1981 ctactatgta gaccacatca acaggaagac gcaatatgaa aacccagtcc tagaagccaa
     2041 acggaagaaa cagcttgaac agcagcagca acagcagcag cctcagccac cgcagccaga
     2101 agagtggaca gaggatcatg catctgttgt gcctcctgtt gctccttccc atcccccgag
     2161 caatccggag ccagccaggg aaactccact tcagggcaaa ccttttttta caagaaaccc
     2221 ctctgagctg aaaggcaagt tcattcacac gaagctacgg aaaagcagcc gaggctttgg
     2281 cttcacggtg gttggaggag acgagcctga tgagttcctg cagatcaaga gcctcgtcct
     2341 cgatggtcct gccgcactgg atggcaagat ggagacaggg gatgtaattg tgagtgtgaa
     2401 tgacacctgt gttttgggac acacacatgc tcaagttgtg aaaatcttcc agtccattcc
     2461 cattggtgcc agtgtggacc ttgaactctg cagaggttat ccattgcctt ttgacccgga
     2521 tgaccctaat acaagtttag tgacctcggt ggccattttg gacaaagaac caattattgt
     2581 aaatggacaa gagacctacg attcaccagc gagccacagt agtaaaacag gcaaagtcag
     2641 cagcatgaag gacgccaggc caagcagccc tgctgatgtg gcttccaaca gctctcatgg
     2701 ttaccccaac gacacagtct ccttggcttc ctccatagcc acccagccag agctaataac
     2761 tgttcacata gtcaaagggc caatgggatt tggctttacg atcgcagaca gtcccggtgg
     2821 gggtggccaa agagtgaaac agattgttga cagtccacgc tgcagaggcc tcaaagaagg
     2881 ggatcttatc gtggaggtga ataagaagaa cgtgcaggcc ctgacgcaca atcaagtcgt
     2941 ggatatgctg attgaatgtc ccaagggaag tgaggtcaca ctgttggtgc agcgaggagg
     3001 gctaccagtt cccaagaaga gcccaaagtc gccactggag aggaaagaca gccagaatag
     3061 ctcccagcac agcgtctcca gccaccggag cctgcacact gcgtccccga gccacggcat
     3121 acaggtgctc cctgagtacc tacctgcaga cgcccctgct ccagatcaga ccgacagctc
     3181 tgggcagaaa aagccagatc cttttaaaat ctgggcccag tccaggagca tgtatgaaaa
     3241 ccgacttcca gattaccagg aacaggacat cttcctctgg agaaaagaaa ccggatttgg
     3301 atttaggatt ctgggtggaa atgaaccagg ggaacccatt tatatcggtc acatcgtacc
     3361 gctgggtgct gctgacacag acggccgcct gaggtctgga gatgaattaa tctgtgtgga
     3421 tgggacacca gtaattggga aatcacacca gctcgtggtc cagcttatgc aacaagctgc
     3481 caagcaaggc catgtcaatc tcacagtgag gcggaaagtg gtctttgccg tccccaaagc
     3541 agagaatgag gtgccctcac cagcctcatc acaccacagt agcaaccagc ccgcgtccct
     3601 gacggaggag aaacgcacac cgcaaggcag ccagaactct ctgaacactg tgagctctgg
     3661 cagcggcagc accagtggca ttggcagtgg tggcggcggg ggcagcggtg tggtgagcgc
     3721 tgtgctgcag ccctatgatg tggagattcg gcgtggggag aacgagggct ttgggtttgt
     3781 catcgtgtcc tccgtgagca ggcccgaagc gggcacaacc ttcgagtcct caaatgccac
     3841 gctgctgact aatgctgaga agattgccac catcaccacc actcatgccc cctctcagca
     3901 ggggacccag gaaacaagga ccaccaccaa accaaagcag gattctcagt ttgagttcaa
     3961 aggaccgcag gctgcacagg agcaagattt ctacactgtg gaattggaaa gaggggccaa
     4021 gggatttggc tttagtcttc gagggggccg agaatataac atggatcttt atgttctgcg
     4081 cttggcagag gatggtcctg cagaaagatg tgggaagatg aggattggcg atgaaattct
     4141 agagatcaat ggtgagacca ccaaaaacat gaaacactct cgggccatag aactgatcaa
     4201 gaatggcggc cgcagggtcc gtctgtttct gcggcgggga gacggctcag tcccagaata
     4261 tgcgatgatc cctcctaaaa tcgctgcatg tatgagaaat gaaaagctcg gggaggcttg
     4321 cttctacctt atgggccata atcaaactac gaccccagca gcgacaggaa cggcccctcc
     4381 accggtgcac aaggtgttcc ggaagtgagg ccggggccgc cagaccacag accgcatcca
     4441 gccttggagt ccagttaccc acccgaactt cacaaatcat cacaacatgc cgaaaagcga
     4501 gcacacgcga aggatccaaa aggcaacagg gagcacagca aacaacccaa cgaacatcac
     4561 acctggaatg gaacttctag aaaaacagga cagcggggca tgccggccca aagaccggcc
     4621 acccgacgca tggagagagg cgcagccaga gcggacagcc accaatggtt caaagaggag
     4681 gtcaccggag aagcgcaggg aaggcacccg cagcgctgac aacactttgg aaagaaggga
     4741 gaagcatgag aagagaagag agatatctcc cgagaggaag cgagagcgtt cacccacccg
     4801 gagaaaagat agctccccga gccgccggcg gaggtccctc gaaagactcc tggatcaaag
     4861 acggtcccca gagcgcagaa gagggggctc ccccgagagg agagccaaat ccaccgacag
     4921 gaggagggcc aggtcccctg agcgcaggcg agagcggtca ctggacaaaa ggaaccggga
     4981 cgacaaggtt ggccaccgag aaagagagga ggctggtctg aagctggaag cggggagaag
     5041 cccccgaaat cccccagagc agagaaggcg gccttacaaa gaatgtagca ccgacctcag
     5101 catctgagac tcagcattgc aacctttacc gtgtcaaagg ttctaagagg aaggtcgcaa
     5161 aacgaaatca tttagtttct tacctgagga agcatctgac acctgatagt cctaagaata
     5221 gtaacgaaca ttttatgcat ctgaaatctt ataaggaaaa atatggaatt ctgcagatat
     5281 ccatcacact ggcggccgct cgagcatgca tctagagggc cctattctat agtgtcacct
     5341 aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     5401 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     5461 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     5521 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     5581 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     5641 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     5701 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     5761 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     5821 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     5881 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     5941 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     6001 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     6061 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     6121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     6181 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     6241 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     6301 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     6361 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     6421 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg
     6481 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     6541 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     6601 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     6661 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     6721 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     6781 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     6841 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     6901 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     6961 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     7021 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     7081 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     7141 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     7201 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     7261 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     7321 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     7381 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     7441 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     7501 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     7561 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     7621 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     7681 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     7741 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     7801 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     7861 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7921 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7981 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8041 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8101 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8161 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8221 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8281 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8341 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     8401 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     8461 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8521 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8581 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     8641 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8701 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8761 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8821 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8881 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8941 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     9001 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     9061 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     9121 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     9181 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9241 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     9301 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     9361 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     9421 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     9481 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     9541 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     9601 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     9661 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9721 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     9781 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgcc
//